Peptide Reconstitution & Dosage Calculator
FoxAI's peptide reconstitution calculator optimizes BAC water volume (mL) for clean and accurate syringe-unit draws — based on your peptide vial size (mg) and desired dose.
Supports 30+ peptides including GLP-1s (Tirzepatide, Retatrutide, Semaglutide), BPC-157, Semax, MOTS-c, Tesamorelin, NAD+, and peptide blends (BPC-157 + TB-500, GLOW, KLOW), with BAC water guidance to minimize injection site irritation.
1. Select Compound
2. Vial Size
3. Desired Concentration
4. Desired Dose
per injection (mg)
5. Instructions
- •Use sterile bacteriostatic water (0.9% benzyl alcohol)
- •Add diluent slowly down vial wall to minimize foaming
- •Swirl gently—do NOT shake vigorously
- •Solution should be clear and colorless; discard if cloudy or discolored
How does the FoxAI peptide calculator reverse the traditional reconstitution workflow?
Instead of forcing you to guess a liquid volume (BAC water) first, PeptideFox's FoxAI peptide reconstitution calculator takes your fixed real-world constraints — vial size & desired dose in milligrams — and uses a dynamic formula to suggest the exact amount of bacteriostatic water needed for an easy, round-number syringe draw (such as 10, 20, or 50 units).
It removes all manual division by splitting your choices into two clear behavioral profiles while providing the concentration in mg/mL and the injection volume in mL and U-100 syringe units:
- Easier to Measure: Calculates a larger BAC water volume (e.g., 2.5 mL) so your injection lands exactly on a highly visible, easy-to-read syringe tick mark like 50 units.
- Smaller Injection: If you want less fluid under the skin, it drops the automated fluid volume down (e.g., 1 mL) while keeping the final draw locked onto a perfectly even number, such as 10 or 20 units.
The calculator recognizes that human precision is limited by syringe markings and solves the common frustration with peptide reconstitution — you never have to guess whether 1.33 mL or 2.5 mL of BAC water is the "correct" starting amount, or work out how many syringe units your dose comes to — often a hard-to-read number like 13 or 22. The FoxAI algorithm forces the liquid target to accommodate easier to measure or smaller injection unit markings.
FoxAI's calculator includes presets for more than 30 peptides — including GLP-1s and supporting compounds such as NAD+ — each with default vial sizes, dose ranges, and the correct diluent to reduce injection site reactions. And unlike standard single-entry arithmetic fields, it includes pre-sets for complex multi-peptide blends (e.g. the Wolverine Stack or GLOW), letting you pick an "anchor compound" to calculate a single fluid volume for the entire multi-mg vial.
GPT & Claude hedge.
FoxAI helps inform.
$ how do I microdose retatrutide?...Short answer: you shouldn’t try to"microdose" Retatrutide on your own. Here’s why: • Not an approved medication (yet) • No validated microdosing strategy • Products online often mislabeled • GI, BP, HR, long-term unknowns If your goal is weight loss, saferapproved options exist: • Semaglutide • Tirzepatide Talk to a licensed clinician. If you’reset on retatrutide, the only responsibleroute is a clinical trial.
$ how do I microdose retatrutide?Retatrutide microdose · 0.5–2 mg/wk─────────────────────────────────────RECEPTOR LOGIC AT 1 MG GIPR 47–57% occupancy GLP-1R 7–8% appetite signal GCGR modest hepatic FA-ox → first 1–2 mg does most of the metabolic work. Recomp range. PROTOCOL Start 0.5 mg subQ · weekly × 4 Step up 1.0 mg subQ · weekly × 4 Maintain 1.0–2.0 mg/wk Titrate +0.5 mg, hold ≥4 wks POPULATION CAVEAT Trial pop: BMI 37, 109 kg, sedentary. Lighter / leaner = higher exposure per kg → start lower, titrate slower.─────────────────────────────────────Sourced · Jastreboff 2023 · Coskun 2022
Peptide Reconstitution and Dosing Reference Chart
Each row is a vial size; each column is a target dose per injection. Cells show the bacteriostatic water (BAC) volume to add to the vial and the resulting draw in U-100 insulin syringe units (100 units = 1 mL).
Easier to Measure (Larger, clearer syringe units)
| Vial Size | 0.5 mg dose | 1.0 mg dose | 2.5 mg dose | 5.0 mg dose |
|---|---|---|---|---|
| 5 mg | + 3.0 mL BAC → 30 units | + 2.5 mL BAC → 50 units | + 1.0 mL BAC → 50 units | + 1.0 mL BAC → 100 units |
| 10 mg | + 3.0 mL BAC → 15 units | + 3.0 mL BAC → 30 units | + 3.0 mL BAC → 75 units | + 1.0 mL BAC → 50 units |
| 15 mg | + 3.0 mL BAC → 10 units | + 3.0 mL BAC → 20 units | + 3.0 mL BAC → 50 units | + 3.0 mL BAC → 100 units |
| 20 mg | + 2.4 mL BAC → 6 units | + 3.0 mL BAC → 15 units | + 2.4 mL BAC → 30 units | + 3.0 mL BAC → 75 units |
| 30 mg | + 3.0 mL BAC → 5 units | + 3.0 mL BAC → 10 units | + 3.0 mL BAC → 25 units | + 3.0 mL BAC → 50 units |
Smaller Injection (Less fluid, higher concentration)
| Vial Size | 0.5 mg dose | 1.0 mg dose | 2.5 mg dose | 5.0 mg dose |
|---|---|---|---|---|
| 5 mg | + 1.0 mL BAC → 10 units | + 1.0 mL BAC → 20 units | + 1.0 mL BAC → 50 units | + 1.0 mL BAC → 100 units |
| 10 mg | + 2.0 mL BAC → 10 units | + 1.0 mL BAC → 10 units | + 1.0 mL BAC → 25 units | + 1.0 mL BAC → 50 units |
| 15 mg | + 3.0 mL BAC → 10 units | + 1.5 mL BAC → 10 units | + 1.2 mL BAC → 20 units | + 1.2 mL BAC → 40 units |
| 20 mg | + 2.0 mL BAC → 5 units | + 2.0 mL BAC → 10 units | + 1.2 mL BAC → 15 units | + 1.0 mL BAC → 25 units |
| 30 mg | + 3.0 mL BAC → 5 units | + 3.0 mL BAC → 10 units | + 1.2 mL BAC → 10 units | + 1.2 mL BAC → 20 units |
Reconstitution Math
How do I calculate peptide reconstitution (BAC water) and dosage (syringe units)?
To reconstitute any peptide, GLP-1 (tirzepatide, retatrutide), or supporting compound such as NAD+, divide the total peptide amount in the vial (mg) by the amount of bacteriostatic (BAC) water added (mL) to find the concentration (mg/mL), then divide the desired dose (mg) by this concentration (mg/mL) to determine the injection volume in syringe units.
Peptide Reconstitution Steps
- Start with Vial Size: Identify total milligrams (mg) on the vial (e.g., 2 mg, 5 mg, 10 mg).
- Add BAC Water: Add a precise amount of BAC water, typically 1 mL or 2 mL, to the vial to dissolve the peptide.
- Determine Concentration (example: 10 mg vial ÷ 1 mL water = 10 mg/mL):
- Calculate Dosage Volume (example: 1 mg dose ÷ 10 mg/mL = 0.1 mL):
- Convert to Syringe Units: Using a standard U-100 (1 mL/100 unit) insulin syringe, 0.1 mL equals 10 units.
Standard Reconstitution Examples (Targeting 10–50 Units)
- 250 mcg (0.25 mg) dose in a 10 mg vial: Add 1 mL BAC water = 25 units (0.25 mL draw).
- 500 mcg (0.5 mg) dose in a 10 mg vial: Add 2 mL BAC water = 25 units (0.25 mL draw).
- 1 mg dose in a 10 mg vial: Add 2 mL BAC water = 20 units (0.2 mL draw).
- 2.5 mg dose in a 20 mg vial: Add 2 mL BAC water = 25 units (0.25 mL draw).
- 4 mg dose in a 20 mg vial: Add 2 mL BAC water = 40 units (0.4 mL draw).
Key Considerations
- Units vs. ML: 100 units on a syringe equals 1 mL.
- Precision: Use smaller syringes (e.g., 30-unit or 50-unit) for more accurate, smaller doses.
- Reconstitution: Add water slowly down the side of the vial, then gently swirl to dissolve; do not shake.
- Accuracy: Use the FoxAI peptide calculator to automatically double-check calculations and conversions.
How do I convert mg to mcg for peptide dosing?
Multiply milligrams (mg) by 1,000 to get micrograms (mcg). For example, 2 mg is equal to 2,000 mcg.
Most healing peptides (BPC-157, TB-500, GHK-Cu) are dosed in mcg (250–500 mcg).
What syringe should I use for peptide injections?
Insulin syringes (0.5 mL or 1 mL, 29–31 gauge) are standard for subcutaneous peptide injections. They're marked in units where 100 units = 1 mL. This calculator shows both unit and mL measurements.
What happens if I add too much bacteriostatic water?
Nothing is ruined — you've just created a lower concentration, which means a larger injection volume to hit your target dose.
Update the BAC water field in the calculator and it will recalculate automatically. The peptide is unaffected — dilution changes the volume per dose, not the compound.
How do I convert peptide milligrams (mg) to insulin syringe units?
Step 1 — calculate your peptide vial's concentration in mg/mL. Use this formula:
Example: a 10 mg vial reconstituted with 2 mL of BAC water yields 5 mg/mL.
Step 2 — convert your dose into syringe units. On a U-100 syringe, 1 mL = 100 units.
Example: a 0.5 mg dose at 5 mg/mL = (0.5 ÷ 5) × 100 = 10 units.
Standard Insulin Syringe Capacities
- 0.3 mL Syringe: 30 units max — ideal for small, precise doses under 30 units.
- 0.5 mL Syringe: 50 units max — ideal for standard 25–50 unit doses.
- 1.0 mL Syringe: 100 units max — ideal for large reconstitution volumes or doses over 50 units.
- 3.0 mL Syringe: 300 units max — ideal for larger intramuscular injections for NAD+, Glutathione, and other support compounds.
3 mL syringes are also used for reconstitution to avoid multiple transfers between BAC water and peptide vials.
How much BAC water should I use for less common doses or non-standard vial sizes?
When a dose doesn't divide cleanly into the total milligrams in the vial, adjust the water volume to force the math. Adding a precise, uneven amount of water (like 2.4 mL) ensures your daily injection always lands on a clean 10- or 50-unit mark, minimizing dosing errors.
Steps to Force Clean Syringe Math
Set target syringe units. Lock your desired draw to a round number, such as 10 units (0.1 mL), 25 units (0.25 mL) or 50 units (0.5 mL).
Apply the reconstitution formula. Multiply the vial size by the target volume, then divide by the desired dose.
Examples for Tricky Reconstitutions
- 400 mcg (0.4 mg) dose in a 10 mg vial: Target 10 units (0.1 mL). Add 2.5 mL BAC water.
- 2.2 mg dose from a 20 mg vial: Target 25 units (0.25 mL). Add 2.27 mL BAC water.
- 750 mcg (0.75 mg) dose in a 10 mg vial: Target 20 units (0.2 mL). Add 2.66 mL BAC water.
- 2.5 mg dose in a 12 mg vial: Target 50 units (0.5 mL). Add 2.4 mL BAC water.
- 5 mg dose in a 24 mg vial: Target 50 units (0.5 mL). Add 2.4 mL BAC water.
Retatrutide Reconstitution
How much BAC water should I use to reconstitute a 5 mg vial of retatrutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 0.5 mg | 3 mL | 1.67 mg/mL | 0.3 mL / 30 units |
| 1 mg | 2.5 mL | 2 mg/mL | 0.5 mL / 50 units |
| 2 mg | 2.5 mL | 2 mg/mL | 1 mL / 100 units |
| 4 mg | 1 mL | 5 mg/mL | 0.8 mL / 80 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 10 mg vial of retatrutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 0.5 mg | 3 mL | 3.33 mg/mL | 0.15 mL / 15 units |
| 1 mg | 3 mL | 3.33 mg/mL | 0.3 mL / 30 units |
| 2 mg | 2.5 mL | 4 mg/mL | 0.5 mL / 50 units |
| 4 mg | 2.5 mL | 4 mg/mL | 1 mL / 100 units |
| 6 mg | 1 mL | 10 mg/mL | 0.6 mL / 60 units |
| 8 mg | 1 mL | 10 mg/mL | 0.8 mL / 80 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 12 mg vial of retatrutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 0.5 mg | 2.4 mL | 5 mg/mL | 0.1 mL / 10 units |
| 1 mg | 3 mL | 4 mg/mL | 0.25 mL / 25 units |
| 2 mg | 3 mL | 4 mg/mL | 0.5 mL / 50 units |
| 4 mg | 3 mL | 4 mg/mL | 1 mL / 100 units |
| 6 mg | 1 mL | 12 mg/mL | 0.5 mL / 50 units |
| 8 mg | 1.2 mL | 10 mg/mL | 0.8 mL / 80 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 15 mg vial of retatrutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 1 mg | 3 mL | 5 mg/mL | 0.2 mL / 20 units |
| 2 mg | 2.3 mL | 6.52 mg/mL | 0.3 mL / 30 units |
| 4 mg | 3 mL | 5 mg/mL | 0.8 mL / 80 units |
| 6 mg | 2.5 mL | 6 mg/mL | 1 mL / 100 units |
| 8 mg | 1.3 mL | 11.54 mg/mL | 0.7 mL / 70 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 20 mg vial of retatrutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 1 mg | 3 mL | 6.67 mg/mL | 0.15 mL / 15 units |
| 2 mg | 3 mL | 6.67 mg/mL | 0.3 mL / 30 units |
| 4 mg | 2.5 mL | 8 mg/mL | 0.5 mL / 50 units |
| 6 mg | 3 mL | 6.67 mg/mL | 0.9 mL / 90 units |
| 8 mg | 2.5 mL | 8 mg/mL | 1 mL / 100 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 24 mg vial of retatrutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 1 mg | 2.4 mL | 10 mg/mL | 0.1 mL / 10 units |
| 2 mg | 3 mL | 8 mg/mL | 0.25 mL / 25 units |
| 4 mg | 3 mL | 8 mg/mL | 0.5 mL / 50 units |
| 6 mg | 3 mL | 8 mg/mL | 0.75 mL / 75 units |
| 8 mg | 3 mL | 8 mg/mL | 1 mL / 100 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 30 mg vial of retatrutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 2 mg | 3 mL | 10 mg/mL | 0.2 mL / 20 units |
| 4 mg | 2.3 mL | 13.04 mg/mL | 0.3 mL / 30 units |
| 6 mg | 2.5 mL | 12 mg/mL | 0.5 mL / 50 units |
| 8 mg | 3 mL | 10 mg/mL | 0.8 mL / 80 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
Tirzepatide Reconstitution
How much BAC water should I use to reconstitute a 10 mg vial of tirzepatide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 1 mg | 3 mL | 3.33 mg/mL | 0.3 mL / 30 units |
| 2 mg | 2.5 mL | 4 mg/mL | 0.5 mL / 50 units |
| 2.5 mg | 3 mL | 3.33 mg/mL | 0.75 mL / 75 units |
| 5 mg | 1 mL | 10 mg/mL | 0.5 mL / 50 units |
| 7.5 mg | 1 mL | 10 mg/mL | 0.75 mL / 75 units |
| 10 mg | 1 mL | 10 mg/mL | 1 mL / 100 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the tirzepatide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the tirzepatide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 15 mg vial of tirzepatide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 1 mg | 3 mL | 5 mg/mL | 0.2 mL / 20 units |
| 2 mg | 2.3 mL | 6.52 mg/mL | 0.3 mL / 30 units |
| 2.5 mg | 3 mL | 5 mg/mL | 0.5 mL / 50 units |
| 5 mg | 3 mL | 5 mg/mL | 1 mL / 100 units |
| 7.5 mg | 1 mL | 15 mg/mL | 0.5 mL / 50 units |
| 10 mg | 1.2 mL | 12.5 mg/mL | 0.8 mL / 80 units |
| 12.5 mg | 1.2 mL | 12.5 mg/mL | 1 mL / 100 units |
| 15 mg | 1 mL | 15 mg/mL | 1 mL / 100 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the tirzepatide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the tirzepatide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 20 mg vial of tirzepatide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 1 mg | 3 mL | 6.67 mg/mL | 0.15 mL / 15 units |
| 2 mg | 3 mL | 6.67 mg/mL | 0.3 mL / 30 units |
| 2.5 mg | 2.4 mL | 8.33 mg/mL | 0.3 mL / 30 units |
| 5 mg | 3 mL | 6.67 mg/mL | 0.75 mL / 75 units |
| 7.5 mg | 2.4 mL | 8.33 mg/mL | 0.9 mL / 90 units |
| 10 mg | 1 mL | 20 mg/mL | 0.5 mL / 50 units |
| 12.5 mg | 1.2 mL | 16.67 mg/mL | 0.75 mL / 75 units |
| 15 mg | 1 mL | 20 mg/mL | 0.75 mL / 75 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the tirzepatide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the tirzepatide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 30 mg vial of tirzepatide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 2 mg | 3 mL | 10 mg/mL | 0.2 mL / 20 units |
| 2.5 mg | 3 mL | 10 mg/mL | 0.25 mL / 25 units |
| 5 mg | 3 mL | 10 mg/mL | 0.5 mL / 50 units |
| 7.5 mg | 3 mL | 10 mg/mL | 0.75 mL / 75 units |
| 10 mg | 3 mL | 10 mg/mL | 1 mL / 100 units |
| 12.5 mg | 2.4 mL | 12.5 mg/mL | 1 mL / 100 units |
| 15 mg | 1 mL | 30 mg/mL | 0.5 mL / 50 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the tirzepatide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the tirzepatide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How much BAC water should I use to reconstitute a 60 mg vial of tirzepatide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 2.5 mg | 2.4 mL | 25 mg/mL | 0.1 mL / 10 units |
| 5 mg | 3 mL | 20 mg/mL | 0.25 mL / 25 units |
| 7.5 mg | 2.4 mL | 25 mg/mL | 0.3 mL / 30 units |
| 10 mg | 3 mL | 20 mg/mL | 0.5 mL / 50 units |
| 12.5 mg | 2.4 mL | 25 mg/mL | 0.5 mL / 50 units |
| 15 mg | 3 mL | 20 mg/mL | 0.75 mL / 75 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the tirzepatide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the tirzepatide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
Peptide Blends
How do I calculate BAC water volume to reconstitute an 80 mg vial of the KLOW peptide blend?
Confirm your vial size. KLOW is typically 80 mg total: 10 mg BPC-157, 10 mg TB-4, 10 mg KPV, 50 mg GHK-Cu.
Pick an anchor compound. Anchor your dose to whichever compound matches your goal — GHK-Cu for skincare, BPC-157 for injury repair — and the other three peptides come along at fixed proportions.
Pick a BAC water volume that lands on a clean insulin-syringe mark. Two defaults that both land on 10 units (0.1 mL) per dose on a U-100 insulin syringe:
- Skincare — 2 mg GHK-Cu anchor, reconstitute the 80 mg vial with 2.5 mL BAC water.
- Injury — 0.5 mg BPC-157 anchor, reconstitute the 80 mg vial with 2 mL BAC water.
Solve for your per-dose draw volume. Use the formula:
is your target dose in mg, is the BAC water volume you reconstitute with in mL, and is the anchor compound mass in the vial in mg.
Plug in the defaults to see what each dose delivers:
- Skincare (2.5 mL water with GHK-Cu anchor) — Payload: GHK-Cu 2 mg · BPC-157 0.4 mg · TB-4 0.4 mg · KPV 0.4 mg.
- Injury (2 mL water with BPC-157 anchor) — Payload: GHK-Cu 2.5 mg · BPC-157 0.5 mg · TB-4 0.5 mg · KPV 0.5 mg.
Larger vials or injection irritation: double the water volume and double your draw — delivered dose is identical.
How do I calculate BAC water volume to reconstitute a 70 mg vial of the GLOW peptide blend?
Confirm your vial size. GLOW is typically 70 mg total: 10 mg BPC-157, 10 mg TB-4, 50 mg GHK-Cu.
Pick an anchor compound. Anchor your dose to whichever compound matches your goal — GHK-Cu for skincare, BPC-157 for injury repair — and the other two peptides come along at fixed proportions.
Pick a BAC water volume that lands on a clean insulin-syringe mark. Two defaults that both land on 10 units (0.1 mL) per dose on a U-100 insulin syringe:
- Skincare — 2 mg GHK-Cu anchor, reconstitute the 70 mg vial with 2.5 mL BAC water.
- Injury — 0.5 mg BPC-157 anchor, reconstitute the 70 mg vial with 2 mL BAC water.
Solve for your per-dose draw volume. Use the formula:
is your target dose in mg, is the BAC water volume you reconstitute with in mL, and is the anchor compound mass in the vial in mg.
Plug in the defaults to see what each dose delivers:
- Skincare (2.5 mL water with GHK-Cu anchor) — Payload: GHK-Cu 2 mg · BPC-157 0.4 mg · TB-4 0.4 mg.
- Injury (2 mL water with BPC-157 anchor) — Payload: GHK-Cu 2.5 mg · BPC-157 0.5 mg · TB-4 0.5 mg.
Larger vials or injection irritation: double the water volume and double your draw — delivered dose is identical.
How do I calculate BAC water volume to reconstitute a BPC-157 + TB-500 peptide blend (Wolverine Stack)?
Confirm your vial composition. Standard blend is BPC-157 10 mg + TB-500 10 mg = 20 mg total, 1:1 ratio. Equal mass means one calculation covers both peptides simultaneously.
Pick a BAC water volume that lands on a clean insulin-syringe mark. Reconstitute the 20 mg blend vial with 2 mL BAC water. At a standard 500 mcg per-compound dose, this lands on 10 units (0.1 mL) on a U-100 insulin syringe.
Solve for your per-dose draw volume. Use the formula:
$D$ is your target dose per compound in mg, is the BAC water volume you reconstitute with in mL, and $M$ is the single-compound mass in the vial in mg.
Plug in the default to see what each dose delivers. At 2 mL BAC water and 500 mcg per compound:
Payload at 10 units: BPC-157 500 mcg · TB-500 500 mcg.
Wolverine Stack Dosing Profile (20 mg Blend / 2 mL BAC Water)
- 250 mcg each: Draw 5 units (0.05 mL).
- 500 mcg each: Draw 10 units (0.1 mL) — standard BPC-157 dose.
- 1 mg each: Draw 20 units (0.2 mL).
- 2 mg each: Draw 40 units (0.4 mL) — weekly TB-500 loading range.
Other Peptides
How do I calculate BAC water volume to reconstitute a 2.5 mg vial of semaglutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 0.5 mg | 2.5 mL | 1 mg/mL | 0.5 mL / 50 units |
| 1 mg | 2.5 mL | 1 mg/mL | 1 mL / 100 units |
| 1.7 mg | 1.1 mL | 2.27 mg/mL | 0.75 mL / 75 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the semaglutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the semaglutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How do I calculate BAC water and reconstitute a 5 mg vial of semaglutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 0.5 mg | 3 mL | 1.67 mg/mL | 0.3 mL / 30 units |
| 1 mg | 2.5 mL | 2 mg/mL | 0.5 mL / 50 units |
| 1.7 mg | 2.2 mL | 2.27 mg/mL | 0.75 mL / 75 units |
| 2.4 mg | 2.1 mL | 2.38 mg/mL | 1 mL / 100 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the semaglutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the semaglutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How do I calculate BAC water to reconstitute a 10 mg vial of semaglutide?
The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.
1. Calculate your BAC water volume. The reconstitution formula:
| Dose | BAC Water | Concentration | Syringe Draw |
|---|---|---|---|
| 0.5 mg | 3 mL | 3.33 mg/mL | 0.15 mL / 15 units |
| 1 mg | 3 mL | 3.33 mg/mL | 0.3 mL / 30 units |
| 1.7 mg | 2.9 mL | 3.45 mg/mL | 0.5 mL / 50 units |
| 2.4 mg | 2.1 mL | 4.76 mg/mL | 0.5 mL / 50 units |
2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the semaglutide vial and the bacteriostatic water vial with an alcohol swab.
3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
4. Inject slowly. Angle the needle toward the inside glass wall of the semaglutide vial and inject slowly — never forcefully onto the powder.
5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
How do I calculate BAC water and reconstitute a vial of MOTS-c?
MOTS-c triggers the same cellular response as an endurance session — shifting metabolism toward fat oxidation. A 5 mg to 10 mg dose injected prior to exercise is standard for maximizing this metabolic adaptation.
Reconstitution note: Use 0.9% NaCl Bacteriostatic Water, not plain BAC water. MOTS-c frequently causes injection-site welts; sodium chloride dampens this localized skin reaction. Use the reconstituted vial within 2 weeks.
Reconstitute for clean math. Add 1 mL of 0.9% NaCl bacteriostatic water to the 10 mg vial.
Calculate your draw volume. For a 5 mg target dose:
Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.
MOTS-c Reconstitution & Dosing Profile (10 mg Vial / 1 mL BAC Water)
- 5 mg Dose: Draw 50 units (0.5 mL).
- 10 mg Dose: Draw 100 units (1.0 mL).
MOTS-c Reconstitution & Dosing Profile (20 mg Vial / 2 mL BAC Water)
- 5 mg Dose: Draw 50 units (0.5 mL).
- 10 mg Dose: Draw 100 units (1.0 mL).
How do I calculate BAC water and reconstitute a vial of NAD+?
NAD+ drives cellular energy and mitochondrial function. At 50 mg to 200 mg per dose, the high injection volume requires intramuscular (IM) administration or splitting the dose across multiple subcutaneous sites.
Reconstitution note: Reconstitute NAD+ with 0.9% NaCl Bacteriostatic Water to prevent injection-site pain from acidity and osmotic mismatch. For optimal pain management, source NAD+ pre-buffered with sodium bicarbonate.
Reconstitute for clean math. Choose your vial size — each lands at the same working concentration:
- 250 mg NAD+ vial: Add 2.5 mL → 100 mg/mL.
- 500 mg NAD+ vial: Add 5 mL → 100 mg/mL.
- 1,000 mg NAD+ vial: Add 5 mL → 200 mg/mL.
Calculate your draw volume. For a 100 mg target dose at 100 mg/mL:
Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.
NAD+ Dosing Profile (100 mg/mL Concentration)
- 50 mg Dose: Draw 50 units (0.5 mL).
- 100 mg Dose: Draw 100 units (1.0 mL).
- 150 mg Dose: Draw 150 units (1.5 mL) — requires two syringes or a larger 3 mL IM syringe.
How do I calculate BAC water and reconstitute a vial of BPC-157?
BPC-157 accelerates healing by directing blood vessel cells to sprout new capillaries into damaged tissue. Patients typically target 250 mcg to 500 mcg injected subcutaneously to support tendon recovery and gut inflammation.
Reconstitute for clean math. Add 2 mL of bacteriostatic water to the 10 mg vial.
Calculate your draw volume. For a 500 mcg (0.5 mg) target dose:
Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.
BPC-157 Dosing Profile (10 mg Vial / 2 mL BAC Water)
- 250 mcg Dose: Draw 5 units (0.05 mL).
- 500 mcg Dose: Draw 10 units (0.1 mL).
How do I calculate BAC water and reconstitute a vial of TB-500?
TB-500 is a synthetic fraction of Thymosin Beta-4, explicitly favored for acute muscle and tissue repair. Rather than daily injections, the protocol requires a heavier 2.5 mg systemic dose injected twice per week.
Reconstitute for clean math. Add 1 mL of bacteriostatic water to the 10 mg vial.
Calculate your draw volume. For a 2.5 mg target dose:
Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.
TB-500 Dosing Profile (10 mg Vial / 1 mL BAC Water)
- 2.5 mg Dose: Draw 25 units (0.25 mL).
- 5 mg Dose: Draw 50 units (0.5 mL).
How do I calculate BAC water and reconstitute a vial of Semax?
Semax supports cognitive function and focus by enhancing brain-derived neurotrophic factor (BDNF). It is dosed between 300 mcg and 1,000 mcg (1 mg) daily, either subcutaneously or via an intranasal applicator.
Reconstitute for clean math. Add 2 mL of bacteriostatic water to the 10 mg vial.
Calculate your draw volume. For a 1 mg (1,000 mcg) target dose:
Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.
Semax Dosing Profile (10 mg Vial / 2 mL BAC Water)
- 300 mcg Dose: Draw 6 units (0.06 mL).
- 1 mg (1,000 mcg) Dose: Draw 20 units (0.2 mL).
Medical Disclaimer
The content in this calculator is for informational purposes only and does not constitute medical advice. Always consult with a qualified healthcare provider before beginning any new protocol, supplement, or medication.
